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sirna targeting ncoa3  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology sirna targeting ncoa3
Expression pattern of <t>NCOA3.</t> (a) Confocal images illustrating the dynamic expression of NCOA3 during human embryogenesis. Hoechst33342 staining (blue) denotes the nucleus. Scale bar = 50 μm. (b) mRNA levels of endogenous NCOA3 during human embryogenesis. Data were analyzed using the comparative Ct (ΔΔCt) method. Mean and S.D. from three independent experiments are presented. * p < 0.05. (c) Confocal images of human blastocysts (left: immunostained for NCOA3; middle: immunostained for CDX2, which is recognized as a marker for trophectoderm; right: overlap). Hoechst33342 staining (blue) denotes the nucleus. Scale bar = 50 μm.
Sirna Targeting Ncoa3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Expression pattern of NCOA3. (a) Confocal images illustrating the dynamic expression of NCOA3 during human embryogenesis. Hoechst33342 staining (blue) denotes the nucleus. Scale bar = 50 μm. (b) mRNA levels of endogenous NCOA3 during human embryogenesis. Data were analyzed using the comparative Ct (ΔΔCt) method. Mean and S.D. from three independent experiments are presented. * p < 0.05. (c) Confocal images of human blastocysts (left: immunostained for NCOA3; middle: immunostained for CDX2, which is recognized as a marker for trophectoderm; right: overlap). Hoechst33342 staining (blue) denotes the nucleus. Scale bar = 50 μm.

Journal: Heliyon

Article Title: NCOA3 knockdown delays human embryo development

doi: 10.1016/j.heliyon.2024.e37639

Figure Lengend Snippet: Expression pattern of NCOA3. (a) Confocal images illustrating the dynamic expression of NCOA3 during human embryogenesis. Hoechst33342 staining (blue) denotes the nucleus. Scale bar = 50 μm. (b) mRNA levels of endogenous NCOA3 during human embryogenesis. Data were analyzed using the comparative Ct (ΔΔCt) method. Mean and S.D. from three independent experiments are presented. * p < 0.05. (c) Confocal images of human blastocysts (left: immunostained for NCOA3; middle: immunostained for CDX2, which is recognized as a marker for trophectoderm; right: overlap). Hoechst33342 staining (blue) denotes the nucleus. Scale bar = 50 μm.

Article Snippet: Approximately 5 % of the zygote/oocyte volume of siRNA targeting NCOA3 (sc-29636; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or scrambled control siRNA (sc-37007; Santa Cruz Biotechnology, Santa Cruz, CA, USA) was injected .

Techniques: Expressing, Staining, Marker

Low levels of NCOA3 indicate low developmental potential of oocytes and embryos. (a) Morphology of day 3 embryos with fragmentation <10 % or >50 %. Scale bar = 100 μm . (b) mRNA level of NCOA3 in day 3 embryos with fragmentation <10 % or >50 %. Data were analyzed using the comparative Ct (ΔΔCt) method. Mean and S.D. from three independent experiments are depicted. *, p < 0.05. (c) Human cleavage-stage embryos developed from 3 PN zygotes were immunostained with an anti-NCOA3 antibody. Hoechst33342 staining (blue) denotes the nucleus. Scale bar = 50 μm. (d) mRNA levels of NCOA3 , NANOG , and OCT4 in oocytes post-injection examined using qPCR and normalized against the levels of ACTB . Data were analyzed using the comparative Ct (ΔΔCt) method. Mean and S.D. from three independent experiments are plotted. *, p < 0.05; **, p < 0.01.

Journal: Heliyon

Article Title: NCOA3 knockdown delays human embryo development

doi: 10.1016/j.heliyon.2024.e37639

Figure Lengend Snippet: Low levels of NCOA3 indicate low developmental potential of oocytes and embryos. (a) Morphology of day 3 embryos with fragmentation <10 % or >50 %. Scale bar = 100 μm . (b) mRNA level of NCOA3 in day 3 embryos with fragmentation <10 % or >50 %. Data were analyzed using the comparative Ct (ΔΔCt) method. Mean and S.D. from three independent experiments are depicted. *, p < 0.05. (c) Human cleavage-stage embryos developed from 3 PN zygotes were immunostained with an anti-NCOA3 antibody. Hoechst33342 staining (blue) denotes the nucleus. Scale bar = 50 μm. (d) mRNA levels of NCOA3 , NANOG , and OCT4 in oocytes post-injection examined using qPCR and normalized against the levels of ACTB . Data were analyzed using the comparative Ct (ΔΔCt) method. Mean and S.D. from three independent experiments are plotted. *, p < 0.05; **, p < 0.01.

Article Snippet: Approximately 5 % of the zygote/oocyte volume of siRNA targeting NCOA3 (sc-29636; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or scrambled control siRNA (sc-37007; Santa Cruz Biotechnology, Santa Cruz, CA, USA) was injected .

Techniques: Staining, Injection

NCOA3 deletion during embryo development. (a) Flow chart. KD, Knockdown. (b) Knockdown efficiency evaluated using qPCR. Data were analyzed using the comparative Ct (ΔΔCt) method. Mean and S.D. from three independent experiments are presented. *, p < 0.05.

Journal: Heliyon

Article Title: NCOA3 knockdown delays human embryo development

doi: 10.1016/j.heliyon.2024.e37639

Figure Lengend Snippet: NCOA3 deletion during embryo development. (a) Flow chart. KD, Knockdown. (b) Knockdown efficiency evaluated using qPCR. Data were analyzed using the comparative Ct (ΔΔCt) method. Mean and S.D. from three independent experiments are presented. *, p < 0.05.

Article Snippet: Approximately 5 % of the zygote/oocyte volume of siRNA targeting NCOA3 (sc-29636; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or scrambled control siRNA (sc-37007; Santa Cruz Biotechnology, Santa Cruz, CA, USA) was injected .

Techniques: Knockdown

Human embryo development following down-regulation of NCOA3 expression in 3 PN zygotes injected with the corresponding siRNA.

Journal: Heliyon

Article Title: NCOA3 knockdown delays human embryo development

doi: 10.1016/j.heliyon.2024.e37639

Figure Lengend Snippet: Human embryo development following down-regulation of NCOA3 expression in 3 PN zygotes injected with the corresponding siRNA.

Article Snippet: Approximately 5 % of the zygote/oocyte volume of siRNA targeting NCOA3 (sc-29636; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or scrambled control siRNA (sc-37007; Santa Cruz Biotechnology, Santa Cruz, CA, USA) was injected .

Techniques: Expressing, Injection

RNA-seq in human oocytes revealed that NCOA3 knockdown affects the expression of many metabolism-related genes. (a) Flow chart of the analysis. (b) Volcano plot showing differentially expressed genes as indicated (circled with a red dotted line). (c) Heatmap illustrating differential up- and down-regulation gene clusters with significant differences (104 genes). (d) KEGG enrichment analysis indicated the enrichment of OxPhos and proteasome pathways.

Journal: Heliyon

Article Title: NCOA3 knockdown delays human embryo development

doi: 10.1016/j.heliyon.2024.e37639

Figure Lengend Snippet: RNA-seq in human oocytes revealed that NCOA3 knockdown affects the expression of many metabolism-related genes. (a) Flow chart of the analysis. (b) Volcano plot showing differentially expressed genes as indicated (circled with a red dotted line). (c) Heatmap illustrating differential up- and down-regulation gene clusters with significant differences (104 genes). (d) KEGG enrichment analysis indicated the enrichment of OxPhos and proteasome pathways.

Article Snippet: Approximately 5 % of the zygote/oocyte volume of siRNA targeting NCOA3 (sc-29636; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or scrambled control siRNA (sc-37007; Santa Cruz Biotechnology, Santa Cruz, CA, USA) was injected .

Techniques: RNA Sequencing, Knockdown, Expressing

Key factors involved in NCOA3 regulation pathways in human oocytes. (a) KDA analysis of differentially expressed genes identify COX7A1 , COX7B , and COX14 as key genes. (b) PPI network depicting the genes that interact directly with NCOA3.

Journal: Heliyon

Article Title: NCOA3 knockdown delays human embryo development

doi: 10.1016/j.heliyon.2024.e37639

Figure Lengend Snippet: Key factors involved in NCOA3 regulation pathways in human oocytes. (a) KDA analysis of differentially expressed genes identify COX7A1 , COX7B , and COX14 as key genes. (b) PPI network depicting the genes that interact directly with NCOA3.

Article Snippet: Approximately 5 % of the zygote/oocyte volume of siRNA targeting NCOA3 (sc-29636; Santa Cruz Biotechnology, Santa Cruz, CA, USA) or scrambled control siRNA (sc-37007; Santa Cruz Biotechnology, Santa Cruz, CA, USA) was injected .

Techniques: